Ambient temperature, but warm to the hand is OK.
It's a known fact that the higher the temperature, the lower the hardness.
Well, here in Sao Paulo, ambient temperature is never lower than 10 Celsius. I don't know what's the ambient temperature where you live. Maybe is minus 30 Celsius, in which case don't let it go down that low for your test. Chances are the material, if carbon steel, will be within the ductile - fragile transition range.
Giovanni S. Crisi
Sao Paulo - Brazil