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Joe, I am not sure how you intend to apply the load. You say "supported on each end with a 1/4" x 5" square tube" so I assume you will not be useing it like boom.
That being said, I should think one would need to know the area (lenght) along the beam the load will be applied as well as distance from the up rights.
Just a thought but if you (assumeing you will be useing a trolly) you can make it wider and spread the load out over a greater distance to effect the deflection.