Calibrate your equipment at 65°C on the EXACT material and all should be good.
The sound velocity varies with the temperature of the test object. That can cause appreciable errors in the measurements if the UT machine was calibrated with a reference block of one temperature and the tested object are a different temperature. Some of the newer flaw detectors have the correction factor built in, or it's possible Henry can find some printed tables online.
A general note is, as the temperature of the test object increases, the sound velocity slightly decreases.
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I have witnessed UT screen displays of cracks in a shaft change in amplitude (higher) when the shaft is under load, and then watched the amplitude fall when the load is removed. And as mentioned heat can have an effect as well. Years ago doing plant thickness baseline surveys, it was common to leave step-wedge calibration blocks on various piping runs for the course of the project so they would be the same temperature as the pipe itself. We also used magnetic thermometers to record the temperature of the pipe for the report.
You bring up an interesting subject. Recently I finished a project where I had to test a valve body with a history of developing fatigue cracks after several months of service. Sure enough, I tested the valve and there was a crack right where the mechanic told me the cracks typically develop. The valve was under pressure and the crack was clearly defines, a nice signal on the display. Life was fine.
The contractor pulled the valve out. It was no easy task; it weighted a couple of tons and had to be moved laterally and they up and out. Long story short, they have me retest it once it was pulled out and the new one installed. No crack! Crank up the gain, no crack!, cranked up the gain to over 100 db, there amongst the back ground you could just make out the signal, but you would miss it if you didn't know where to find it.
An engineer suggested heating the valve with a torch. Differential heating, damn, there was the crack, clear as day, proud as a new feather. Just goes to show you, your never too old to learn something new.
Best regards - Al